Pensacola Little Theatre proclaims itself to be "the oldest continually-producing community theatre in the Southeastern United States." PLT formed in the mid-1930s, as part of the WPA project to revitalize the country following the depression. From a handful of shows in 1936, the theatre has continued to expand its offerings and now puts on nearly two-dozen stage shows every year, ranging from Broadway musicals to classic dramas to original plays by local authors. Aside from the small management and office staff, everyone who works at PLT is a volunteer. None of the actors and crew members get paid for the weeks of work they put in to bring a show to the stage.

Pensacola Little Theatre offers three distinct series:

The MainStage series is five shows, usually well-known titles, award-winning classics, and nothing too offensive. 

Treehouse is a series of three shows intended to be enjoyed by all ages. Sometimes the casts are all children and teenages, other times adults are used, but the fare is always appropriate for children.

Studio 400 offers four productions that really push the envelope. They include controversial topics, mature themes, and adult language. This series is also the most likely place to see locally written material. These shows are performed in a banquet hall known as "The Courtroom."

In addition to the established series, Pensacola Little Theatre offers several "Special Productions" each season. These are shows, usually presented on the MainStage, which fall outside the parameteres of the established series. They also serve as popular fundraisers for the not-for-profit theatre.

As a non-profit, Pensacola Little Theatre also produces an annual gala fundraiser "Life is a Cabaret" that turns the entire three stories of PLT's building, the Pensacola Cultural Center, into one big party, organized around a central theme such as movies or rock music. Every other year, PLT puts on "Stage Fright," a haunted house fundraiser, which again makes use of the entire building.